Feeblin Options
Three plasmids (pGL1124, pGL1224 and pGL1217) were made to enable the alternative of 1 allele of CYC9Lister 427 cells. Trypanosomes had been transfected with Each and every plasmid independently, and after that subjected into a 2nd transfection with one of several other plasmids, with a different resistance gene, with all pairwise mixtures carried